Construct: shRNA TRCN0000287805

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009679.2-768s21c1
DNA Barcode:
GCCTGAGTGCAAGTTTGGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ap2m1 (11773)
Gene Description:
adaptor-related protein complex 2, mu 1 subunit
Transcript:
RefSeq NM_009679.2 (NON-CURRENT)
Match location:
Position 768 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000287805)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGAGTGCAAGTTTGGAAT
Hairpin Sequence:
5'-CCGG-GCCTGAGTGCAAGTTTGGAAT-CTCGAG-ATTCCAAACTTGCACTCAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGAGTGCAAGTTTGGAATCTCGAGATTCCAAACTTGCACTCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGAGTGCAAGTTTGGAATCTCGAGATTCCAAACTTGCACTCAGGC-3'

Other clones with same target sequence:

  1. TRCN0000111748