Construct: shRNA TRCN0000288089

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207649.1-294s21c1
DNA Barcode:
CAGGCATCTATCCCTGAAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rcan2 (53901)
Gene Description:
regulator of calcineurin 2
Transcript:
RefSeq NM_207649.1 (CURRENT)
Match location:
Position 294 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000288089)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGCATCTATCCCTGAAGAT
Hairpin Sequence:
5'-CCGG-CAGGCATCTATCCCTGAAGAT-CTCGAG-ATCTTCAGGGATAGATGCCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGCATCTATCCCTGAAGATCTCGAGATCTTCAGGGATAGATGCCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGCATCTATCCCTGAAGATCTCGAGATCTTCAGGGATAGATGCCTG-3'

Other clones with same target sequence:

  1. TRCN0000105806