Construct: shRNA TRCN0000288198

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026319.2-964s21c1
DNA Barcode:
GAGGCTGTATTGCTGTATGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ift74 (67694)
Gene Description:
intraflagellar transport 74
Transcript:
RefSeq NM_026319.2 (NON-CURRENT)
Match location:
Position 964 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000288198)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGCTGTATTGCTGTATGAA
Hairpin Sequence:
5'-CCGG-GAGGCTGTATTGCTGTATGAA-CTCGAG-TTCATACAGCAATACAGCCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGCTGTATTGCTGTATGAACTCGAGTTCATACAGCAATACAGCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGCTGTATTGCTGTATGAACTCGAGTTCATACAGCAATACAGCCTC-3'

Other clones with same target sequence:

  1. TRCN0000113229