Construct: shRNA TRCN0000288207

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026876.2-2427s21c1
DNA Barcode:
GTTCTTCTCAGGGTTCTGTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trmt1l (98685)
Gene Description:
tRNA methyltransferase 1 like
Transcript:
RefSeq NM_026876.2 (NON-CURRENT)
Match location:
Position 2427 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000288207)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCTTCTCAGGGTTCTGTAT
Hairpin Sequence:
5'-CCGG-GTTCTTCTCAGGGTTCTGTAT-CTCGAG-ATACAGAACCCTGAGAAGAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCTTCTCAGGGTTCTGTATCTCGAGATACAGAACCCTGAGAAGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCTTCTCAGGGTTCTGTATCTCGAGATACAGAACCCTGAGAAGAAC-3'

Other clones with same target sequence:

  1. TRCN0000126964