Construct: shRNA TRCN0000288265

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024431.2-965s21c1
DNA Barcode:
GCGCCACATTTGCTGAGATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Morf4l1 (21761)
Gene Description:
mortality factor 4 like 1
Transcript:
RefSeq NM_024431.2 (NON-CURRENT)
Match location:
Position 965 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000288265)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCCACATTTGCTGAGATTA
Hairpin Sequence:
5'-CCGG-GCGCCACATTTGCTGAGATTA-CTCGAG-TAATCTCAGCAAATGTGGCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCCACATTTGCTGAGATTACTCGAGTAATCTCAGCAAATGTGGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCCACATTTGCTGAGATTACTCGAGTAATCTCAGCAAATGTGGCGC-3'

Other clones with same target sequence:

  1. TRCN0000071527