Construct: shRNA TRCN0000288304

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026849.2-978s21c1
DNA Barcode:
CCTGACTCAATCTCTGAACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mtmr14 (97287)
Gene Description:
myotubularin related protein 14
Transcript:
RefSeq NM_026849.2 (CURRENT)
Match location:
Position 978 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000288304)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGACTCAATCTCTGAACAT
Hairpin Sequence:
5'-CCGG-CCTGACTCAATCTCTGAACAT-CTCGAG-ATGTTCAGAGATTGAGTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGACTCAATCTCTGAACATCTCGAGATGTTCAGAGATTGAGTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGACTCAATCTCTGAACATCTCGAGATGTTCAGAGATTGAGTCAGG-3'

Other clones with same target sequence:

  1. TRCN0000080741