Construct: shRNA TRCN0000288335

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145470.2-994s21c1
DNA Barcode:
GCAAGGAAGACATTCACGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Deptor (97998)
Gene Description:
DEP domain containing MTOR-interacting protein
Transcript:
RefSeq NM_145470.2 (NON-CURRENT)
Match location:
Position 994 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000288335)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAGGAAGACATTCACGATT
Hairpin Sequence:
5'-CCGG-GCAAGGAAGACATTCACGATT-CTCGAG-AATCGTGAATGTCTTCCTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAGGAAGACATTCACGATTCTCGAGAATCGTGAATGTCTTCCTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAGGAAGACATTCACGATTCTCGAGAATCGTGAATGTCTTCCTTGC-3'

Other clones with same target sequence:

  1. TRCN0000110159
  2. TRCN0000168393