Construct: shRNA TRCN0000288398

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023884.3-274s21c1
DNA Barcode:
CGCGGGTCAGATAACACTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ralgps2 (78255)
Gene Description:
Ral GEF with PH domain and SH3 binding motif 2
Transcript:
RefSeq NM_023884.3 (NON-CURRENT)
Match location:
Position 274 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000288398)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCGGGTCAGATAACACTAAT
Hairpin Sequence:
5'-CCGG-CGCGGGTCAGATAACACTAAT-CTCGAG-ATTAGTGTTATCTGACCCGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCGGGTCAGATAACACTAATCTCGAGATTAGTGTTATCTGACCCGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCGGGTCAGATAACACTAATCTCGAGATTAGTGTTATCTGACCCGCG-3'

Other clones with same target sequence:

  1. TRCN0000110113