Construct: shRNA TRCN0000288407

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_174995.2-264s21c1
DNA Barcode:
GCTGGGTGGTACTTCAATCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mgst2 (211666)
Gene Description:
microsomal glutathione S-transferase 2
Transcript:
RefSeq NM_174995.2 (NON-CURRENT)
Match location:
Position 264 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000288407)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGGTGGTACTTCAATCAA
Hairpin Sequence:
5'-CCGG-GCTGGGTGGTACTTCAATCAA-CTCGAG-TTGATTGAAGTACCACCCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGGTGGTACTTCAATCAACTCGAGTTGATTGAAGTACCACCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGGTGGTACTTCAATCAACTCGAGTTGATTGAAGTACCACCCAGC-3'

Other clones with same target sequence:

  1. TRCN0000103306