Construct: shRNA TRCN0000288455

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023144.1-283s21c1
DNA Barcode:
GCCAGAATGAAGGCTTGACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nono (53610)
Gene Description:
non-POU-domain-containing, octamer binding protein
Transcript:
RefSeq NM_023144.1 (NON-CURRENT)
Match location:
Position 283 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000288455)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGAATGAAGGCTTGACTA
Hairpin Sequence:
5'-CCGG-GCCAGAATGAAGGCTTGACTA-CTCGAG-TAGTCAAGCCTTCATTCTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGAATGAAGGCTTGACTACTCGAGTAGTCAAGCCTTCATTCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGAATGAAGGCTTGACTACTCGAGTAGTCAAGCCTTCATTCTGGC-3'

Other clones with same target sequence:

  1. TRCN0000102627