Construct: shRNA TRCN0000288479

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133916.2-2534s21c1
DNA Barcode:
GCTGGGCTCTTACCTGTGCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Eif3b (27979)
Gene Description:
eukaryotic translation initiation factor 3, subunit B
Transcript:
RefSeq NM_133916.2 (CURRENT)
Match location:
Position 2534 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000288479)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGGCTCTTACCTGTGCTT
Hairpin Sequence:
5'-CCGG-GCTGGGCTCTTACCTGTGCTT-CTCGAG-AAGCACAGGTAAGAGCCCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGGCTCTTACCTGTGCTTCTCGAGAAGCACAGGTAAGAGCCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGGCTCTTACCTGTGCTTCTCGAGAAGCACAGGTAAGAGCCCAGC-3'

Other clones with same target sequence:

  1. TRCN0000096909