Construct: shRNA TRCN0000288497

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172845.2-2798s21c1
DNA Barcode:
CGTCTGCGGTACAGTTTCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adamts4 (240913)
Gene Description:
a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 4
Transcript:
RefSeq NM_172845.2 (NON-CURRENT)
Match location:
Position 2798 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000288497)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCTGCGGTACAGTTTCTTT
Hairpin Sequence:
5'-CCGG-CGTCTGCGGTACAGTTTCTTT-CTCGAG-AAAGAAACTGTACCGCAGACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCTGCGGTACAGTTTCTTTCTCGAGAAAGAAACTGTACCGCAGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCTGCGGTACAGTTTCTTTCTCGAGAAAGAAACTGTACCGCAGACG-3'

Other clones with same target sequence:

  1. TRCN0000032008