Construct: shRNA TRCN0000288499

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008249.4-258s21c1
DNA Barcode:
GCGGCTTCTCTGACTTCAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tfb2m (15278)
Gene Description:
transcription factor B2, mitochondrial
Transcript:
RefSeq NM_008249.4 (NON-CURRENT)
Match location:
Position 258 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000288499)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGCTTCTCTGACTTCAATA
Hairpin Sequence:
5'-CCGG-GCGGCTTCTCTGACTTCAATA-CTCGAG-TATTGAAGTCAGAGAAGCCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGCTTCTCTGACTTCAATACTCGAGTATTGAAGTCAGAGAAGCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGCTTCTCTGACTTCAATACTCGAGTATTGAAGTCAGAGAAGCCGC-3'

Other clones with same target sequence:

  1. TRCN0000081604