Construct: shRNA TRCN0000288516

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009357.2-215s21c1
DNA Barcode:
CCGAATCATCAAATACATGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tex261 (21766)
Gene Description:
testis expressed gene 261
Transcript:
RefSeq NM_009357.2 (CURRENT)
Match location:
Position 215 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000288516)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAATCATCAAATACATGAT
Hairpin Sequence:
5'-CCGG-CCGAATCATCAAATACATGAT-CTCGAG-ATCATGTATTTGATGATTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAATCATCAAATACATGATCTCGAGATCATGTATTTGATGATTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAATCATCAAATACATGATCTCGAGATCATGTATTTGATGATTCGG-3'

Other clones with same target sequence:

  1. TRCN0000126275