Construct: shRNA TRCN0000288539

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009462.1-1621s21c1
DNA Barcode:
CGCAGAGGAGTATCTAGGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Usp10 (22224)
Gene Description:
ubiquitin specific peptidase 10
Transcript:
RefSeq NM_009462.1 (NON-CURRENT)
Match location:
Position 1621 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000288539)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCAGAGGAGTATCTAGGTTT
Hairpin Sequence:
5'-CCGG-CGCAGAGGAGTATCTAGGTTT-CTCGAG-AAACCTAGATACTCCTCTGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCAGAGGAGTATCTAGGTTTCTCGAGAAACCTAGATACTCCTCTGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCAGAGGAGTATCTAGGTTTCTCGAGAAACCTAGATACTCCTCTGCG-3'

Other clones with same target sequence:

  1. TRCN0000030766