Construct: shRNA TRCN0000288545

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011396.3-1107s21c1
DNA Barcode:
CCAAGTGAGTTACAAGACTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc22a5 (20520)
Gene Description:
solute carrier family 22 (organic cation transporter), member 5
Transcript:
RefSeq NM_011396.3 (CURRENT)
Match location:
Position 1107 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000288545)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGTGAGTTACAAGACTTA
Hairpin Sequence:
5'-CCGG-CCAAGTGAGTTACAAGACTTA-CTCGAG-TAAGTCTTGTAACTCACTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGTGAGTTACAAGACTTACTCGAGTAAGTCTTGTAACTCACTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGTGAGTTACAAGACTTACTCGAGTAAGTCTTGTAACTCACTTGG-3'

Other clones with same target sequence:

  1. TRCN0000070220