Construct: shRNA TRCN0000288628

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014705.3-4944s21c1
DNA Barcode:
GCCGTGCATGAGAAGTTTGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DOCK4 (9732)
Gene Description:
dedicator of cytokinesis 4
Transcript:
RefSeq NM_014705.3 (NON-CURRENT)
Match location:
Position 4944 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000288628)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGTGCATGAGAAGTTTGTA
Hairpin Sequence:
5'-CCGG-GCCGTGCATGAGAAGTTTGTA-CTCGAG-TACAAACTTCTCATGCACGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGTGCATGAGAAGTTTGTACTCGAGTACAAACTTCTCATGCACGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGTGCATGAGAAGTTTGTACTCGAGTACAAACTTCTCATGCACGGC-3'

Other clones with same target sequence:

  1. TRCN0000039730