Construct: shRNA TRCN0000288683

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004586.2-1096s21c1
DNA Barcode:
CCCAAAGATTCACCTGGCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPS6KA3 (6197)
Gene Description:
ribosomal protein S6 kinase A3
Transcript:
RefSeq NM_004586.2 (NON-CURRENT)
Match location:
Position 1096 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000288683)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAAAGATTCACCTGGCATT
Hairpin Sequence:
5'-CCGG-CCCAAAGATTCACCTGGCATT-CTCGAG-AATGCCAGGTGAATCTTTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAAAGATTCACCTGGCATTCTCGAGAATGCCAGGTGAATCTTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAAAGATTCACCTGGCATTCTCGAGAATGCCAGGTGAATCTTTGGG-3'

Other clones with same target sequence:

  1. TRCN0000040145