Construct: shRNA TRCN0000288750

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005787.5-1039s21c1
DNA Barcode:
CCAGATCGTTTCTACCCTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ALG3 (10195)
Gene Description:
ALG3 alpha-1,3- mannosyltransferase
Transcript:
RefSeq NM_005787.5 (NON-CURRENT)
Match location:
Position 1039 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000288750)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGATCGTTTCTACCCTCTT
Hairpin Sequence:
5'-CCGG-CCAGATCGTTTCTACCCTCTT-CTCGAG-AAGAGGGTAGAAACGATCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGATCGTTTCTACCCTCTTCTCGAGAAGAGGGTAGAAACGATCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGATCGTTTCTACCCTCTTCTCGAGAAGAGGGTAGAAACGATCTGG-3'

Other clones with same target sequence:

  1. TRCN0000035444