Construct: shRNA TRCN0000288909

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002245.3-1430s21c1
DNA Barcode:
CGACCTTACATAGGAGGAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCNK1 (3775)
Gene Description:
potassium two pore domain channel subfamily K member 1
Transcript:
RefSeq NM_002245.3 (NON-CURRENT)
Match location:
Position 1430 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000288909)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACCTTACATAGGAGGAGAA
Hairpin Sequence:
5'-CCGG-CGACCTTACATAGGAGGAGAA-CTCGAG-TTCTCCTCCTATGTAAGGTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACCTTACATAGGAGGAGAACTCGAGTTCTCCTCCTATGTAAGGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACCTTACATAGGAGGAGAACTCGAGTTCTCCTCCTATGTAAGGTCG-3'

Other clones with same target sequence:

  1. TRCN0000044723