Construct: shRNA TRCN0000288914
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_175066.3-624s21c1
- DNA Barcode:
- CCTGGTTCCTATCGAGGACAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DDX51 (317781)
- Gene Description:
- DEAD-box helicase 51
- Transcript:
- RefSeq NM_175066.3 (NON-CURRENT)
- Match location:
- Position 624 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCTGGTTCCTATCGAGGACAT
- Hairpin Sequence:
-
5'-CCGG-CCTGGTTCCTATCGAGGACAT-CTCGAG-ATGTCCTCGATAGGAACCAGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCTGGTTCCTATCGAGGACATCTCGAGATGTCCTCGATAGGAACCAGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCTGGTTCCTATCGAGGACATCTCGAGATGTCCTCGATAGGAACCAGG-3'
Other clones with same target sequence:
-
TRCN0000050002
