Construct: shRNA TRCN0000288927

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014331.3-544s21c1
DNA Barcode:
CCTGTCACTATTTGGAGCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC7A11 (23657)
Gene Description:
solute carrier family 7 member 11
Transcript:
RefSeq NM_014331.3 (NON-CURRENT)
Match location:
Position 544 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000288927)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTCACTATTTGGAGCTTT
Hairpin Sequence:
5'-CCGG-CCTGTCACTATTTGGAGCTTT-CTCGAG-AAAGCTCCAAATAGTGACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTCACTATTTGGAGCTTTCTCGAGAAAGCTCCAAATAGTGACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTCACTATTTGGAGCTTTCTCGAGAAAGCTCCAAATAGTGACAGG-3'

Other clones with same target sequence:

  1. TRCN0000043123