Construct: shRNA TRCN0000288938

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002053.2-899s21c1
DNA Barcode:
CGGAAATTCTTCCCAAAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GBP1 (2633)
Gene Description:
guanylate binding protein 1
Transcript:
RefSeq NM_002053.2 (NON-CURRENT)
Match location:
Position 899 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000288938)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAAATTCTTCCCAAAGAAA
Hairpin Sequence:
5'-CCGG-CGGAAATTCTTCCCAAAGAAA-CTCGAG-TTTCTTTGGGAAGAATTTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAAATTCTTCCCAAAGAAACTCGAGTTTCTTTGGGAAGAATTTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAAATTCTTCCCAAAGAAACTCGAGTTTCTTTGGGAAGAATTTCCG-3'

Other clones with same target sequence:

  1. TRCN0000116118