Construct: shRNA TRCN0000288941

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005482.2-628s21c1
DNA Barcode:
CGCTACAATGAGCTACTGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIGK (10026)
Gene Description:
phosphatidylinositol glycan anchor biosynthesis class K
Transcript:
RefSeq NM_005482.2 (NON-CURRENT)
Match location:
Position 628 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000288941)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTACAATGAGCTACTGTTT
Hairpin Sequence:
5'-CCGG-CGCTACAATGAGCTACTGTTT-CTCGAG-AAACAGTAGCTCATTGTAGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTACAATGAGCTACTGTTTCTCGAGAAACAGTAGCTCATTGTAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTACAATGAGCTACTGTTTCTCGAGAAACAGTAGCTCATTGTAGCG-3'

Other clones with same target sequence:

  1. TRCN0000050120