Construct: shRNA TRCN0000288963

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004408.2-993s21c1
DNA Barcode:
CCAGCTACTGTCCATTGAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DNM1 (1759)
Gene Description:
dynamin 1
Transcript:
RefSeq NM_004408.2 (NON-CURRENT)
Match location:
Position 993 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000288963)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCTACTGTCCATTGAGAA
Hairpin Sequence:
5'-CCGG-CCAGCTACTGTCCATTGAGAA-CTCGAG-TTCTCAATGGACAGTAGCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCTACTGTCCATTGAGAACTCGAGTTCTCAATGGACAGTAGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCTACTGTCCATTGAGAACTCGAGTTCTCAATGGACAGTAGCTGG-3'

Other clones with same target sequence:

  1. TRCN0000051771