Construct: shRNA TRCN0000289000

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003088.2-1170s21c1
DNA Barcode:
CAAGTTTGTGACCTCCAAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FSCN1 (6624)
Gene Description:
fascin actin-bundling protein 1
Transcript:
RefSeq NM_003088.2 (NON-CURRENT)
Match location:
Position 1170 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000289000)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGTTTGTGACCTCCAAGAA
Hairpin Sequence:
5'-CCGG-CAAGTTTGTGACCTCCAAGAA-CTCGAG-TTCTTGGAGGTCACAAACTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGTTTGTGACCTCCAAGAACTCGAGTTCTTGGAGGTCACAAACTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGTTTGTGACCTCCAAGAACTCGAGTTCTTGGAGGTCACAAACTTG-3'

Other clones with same target sequence:

  1. TRCN0000123042