Construct: shRNA TRCN0000289002

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001233.3-946s21c1
DNA Barcode:
GCACCACTGTTCTGTTCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAV2 (858)
Gene Description:
caveolin 2
Transcript:
RefSeq NM_001233.3 (NON-CURRENT)
Match location:
Position 946 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000289002)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACCACTGTTCTGTTCATTT
Hairpin Sequence:
5'-CCGG-GCACCACTGTTCTGTTCATTT-CTCGAG-AAATGAACAGAACAGTGGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACCACTGTTCTGTTCATTTCTCGAGAAATGAACAGAACAGTGGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACCACTGTTCTGTTCATTTCTCGAGAAATGAACAGAACAGTGGTGC-3'

Other clones with same target sequence:

  1. TRCN0000123019