Construct: shRNA TRCN0000289009

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002627.3-1207s21c1
DNA Barcode:
CTGAACACCTACAAGCGACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PFKP (5214)
Gene Description:
phosphofructokinase, platelet
Transcript:
RefSeq NM_002627.3 (NON-CURRENT)
Match location:
Position 1207 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000289009)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGAACACCTACAAGCGACTT
Hairpin Sequence:
5'-CCGG-CTGAACACCTACAAGCGACTT-CTCGAG-AAGTCGCTTGTAGGTGTTCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGAACACCTACAAGCGACTTCTCGAGAAGTCGCTTGTAGGTGTTCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGAACACCTACAAGCGACTTCTCGAGAAGTCGCTTGTAGGTGTTCAG-3'

Other clones with same target sequence:

  1. TRCN0000037777