Construct: shRNA TRCN0000289015

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002647.2-2183s21c1
DNA Barcode:
CCAAGTGAGAATGGGCCAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIK3C3 (5289)
Gene Description:
phosphatidylinositol 3-kinase catalytic subunit type 3
Transcript:
RefSeq NM_002647.2 (NON-CURRENT)
Match location:
Position 2183 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000289015)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGTGAGAATGGGCCAAAT
Hairpin Sequence:
5'-CCGG-CCAAGTGAGAATGGGCCAAAT-CTCGAG-ATTTGGCCCATTCTCACTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGTGAGAATGGGCCAAATCTCGAGATTTGGCCCATTCTCACTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGTGAGAATGGGCCAAATCTCGAGATTTGGCCCATTCTCACTTGG-3'

Other clones with same target sequence:

  1. TRCN0000037795