Construct: shRNA TRCN0000289085

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004148.3-371s21c1
DNA Barcode:
CCTTGTCAAGTACGACCTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NINJ1 (4814)
Gene Description:
ninjurin 1
Transcript:
RefSeq NM_004148.3 (NON-CURRENT)
Match location:
Position 371 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000289085)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTGTCAAGTACGACCTTAA
Hairpin Sequence:
5'-CCGG-CCTTGTCAAGTACGACCTTAA-CTCGAG-TTAAGGTCGTACTTGACAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTGTCAAGTACGACCTTAACTCGAGTTAAGGTCGTACTTGACAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTGTCAAGTACGACCTTAACTCGAGTTAAGGTCGTACTTGACAAGG-3'

Other clones with same target sequence:

  1. TRCN0000063768