Construct: shRNA TRCN0000289128

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016448.2-1570s21c1
DNA Barcode:
GCCTAGTAACAGTAACGAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DTL (51514)
Gene Description:
denticleless E3 ubiquitin protein ligase homolog
Transcript:
RefSeq NM_016448.2 (NON-CURRENT)
Match location:
Position 1570 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000289128)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTAGTAACAGTAACGAGTA
Hairpin Sequence:
5'-CCGG-GCCTAGTAACAGTAACGAGTA-CTCGAG-TACTCGTTACTGTTACTAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTAGTAACAGTAACGAGTACTCGAGTACTCGTTACTGTTACTAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTAGTAACAGTAACGAGTACTCGAGTACTCGTTACTGTTACTAGGC-3'

Other clones with same target sequence:

  1. TRCN0000118813