Construct: shRNA TRCN0000289129

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000337.5-998s21c1
DNA Barcode:
GAGCTGAAAGATTACGAGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SGCD (6444)
Gene Description:
sarcoglycan delta
Transcript:
RefSeq NM_000337.5 (CURRENT)
Match location:
Position 998 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000289129)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCTGAAAGATTACGAGTTT
Hairpin Sequence:
5'-CCGG-GAGCTGAAAGATTACGAGTTT-CTCGAG-AAACTCGTAATCTTTCAGCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCTGAAAGATTACGAGTTTCTCGAGAAACTCGTAATCTTTCAGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCTGAAAGATTACGAGTTTCTCGAGAAACTCGTAATCTTTCAGCTC-3'

Other clones with same target sequence:

  1. TRCN0000118875