Construct: shRNA TRCN0000289212

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020214.2-1260s21c1
DNA Barcode:
CCTCGTTCAGATCATGAAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PARP6 (56965)
Gene Description:
poly(ADP-ribose) polymerase family member 6
Transcript:
RefSeq NM_020214.2 (NON-CURRENT)
Match location:
Position 1260 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000289212)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCGTTCAGATCATGAAGTA
Hairpin Sequence:
5'-CCGG-CCTCGTTCAGATCATGAAGTA-CTCGAG-TACTTCATGATCTGAACGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCGTTCAGATCATGAAGTACTCGAGTACTTCATGATCTGAACGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCGTTCAGATCATGAAGTACTCGAGTACTTCATGATCTGAACGAGG-3'

Other clones with same target sequence:

  1. TRCN0000053207