Construct: shRNA TRCN0000289215

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025205.3-445s21c1
DNA Barcode:
GACATCAACGTGCAGCACAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MED28 (80306)
Gene Description:
mediator complex subunit 28
Transcript:
RefSeq NM_025205.3 (NON-CURRENT)
Match location:
Position 445 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000289215)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACATCAACGTGCAGCACAAA
Hairpin Sequence:
5'-CCGG-GACATCAACGTGCAGCACAAA-CTCGAG-TTTGTGCTGCACGTTGATGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACATCAACGTGCAGCACAAACTCGAGTTTGTGCTGCACGTTGATGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACATCAACGTGCAGCACAAACTCGAGTTTGTGCTGCACGTTGATGTC-3'

Other clones with same target sequence:

  1. TRCN0000053235