Construct: shRNA TRCN0000289261

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004311.2-516s21c1
DNA Barcode:
CCAGTGCTCATCTTTGCTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARL3 (403)
Gene Description:
ADP ribosylation factor like GTPase 3
Transcript:
RefSeq NM_004311.2 (NON-CURRENT)
Match location:
Position 516 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000289261)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTGCTCATCTTTGCTAAT
Hairpin Sequence:
5'-CCGG-CCAGTGCTCATCTTTGCTAAT-CTCGAG-ATTAGCAAAGATGAGCACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTGCTCATCTTTGCTAATCTCGAGATTAGCAAAGATGAGCACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTGCTCATCTTTGCTAATCTCGAGATTAGCAAAGATGAGCACTGG-3'

Other clones with same target sequence:

  1. TRCN0000048035