Construct: shRNA TRCN0000289267

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005716.2-1699s21c1
DNA Barcode:
GCAAATGCAATAATGCCCTCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GIPC1 (10755)
Gene Description:
GIPC PDZ domain containing family member 1
Transcript:
RefSeq NM_005716.2 (NON-CURRENT)
Match location:
Position 1699 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000289267)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAATGCAATAATGCCCTCA
Hairpin Sequence:
5'-CCGG-GCAAATGCAATAATGCCCTCA-CTCGAG-TGAGGGCATTATTGCATTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAATGCAATAATGCCCTCACTCGAGTGAGGGCATTATTGCATTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAATGCAATAATGCCCTCACTCGAGTGAGGGCATTATTGCATTTGC-3'

Other clones with same target sequence:

  1. TRCN0000036769