Construct: shRNA TRCN0000289292

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024615.2-1244s21c1
DNA Barcode:
GCCTAACTCTAAAGTCGCATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PARP8 (79668)
Gene Description:
poly(ADP-ribose) polymerase family member 8
Transcript:
RefSeq NM_024615.2 (NON-CURRENT)
Match location:
Position 1244 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000289292)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTAACTCTAAAGTCGCATA
Hairpin Sequence:
5'-CCGG-GCCTAACTCTAAAGTCGCATA-CTCGAG-TATGCGACTTTAGAGTTAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTAACTCTAAAGTCGCATACTCGAGTATGCGACTTTAGAGTTAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTAACTCTAAAGTCGCATACTCGAGTATGCGACTTTAGAGTTAGGC-3'

Other clones with same target sequence:

  1. TRCN0000053224