Construct: shRNA TRCN0000289318

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000676.2-926s21c1
DNA Barcode:
GCTGGTGATCTACATTAAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADORA2B (136)
Gene Description:
adenosine A2b receptor
Transcript:
RefSeq NM_000676.2 (CURRENT)
Match location:
Position 926 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000289318)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGTGATCTACATTAAGAT
Hairpin Sequence:
5'-CCGG-GCTGGTGATCTACATTAAGAT-CTCGAG-ATCTTAATGTAGATCACCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGTGATCTACATTAAGATCTCGAGATCTTAATGTAGATCACCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGTGATCTACATTAAGATCTCGAGATCTTAATGTAGATCACCAGC-3'

Other clones with same target sequence:

  1. TRCN0000065334