Construct: shRNA TRCN0000289389

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013338.4-399s21c1
DNA Barcode:
GCAGTAAAGATCAGACCTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ALG5 (29880)
Gene Description:
ALG5 dolichyl-phosphate beta-glucosyltransferase
Transcript:
RefSeq NM_013338.4 (NON-CURRENT)
Match location:
Position 399 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000289389)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTAAAGATCAGACCTCAA
Hairpin Sequence:
5'-CCGG-GCAGTAAAGATCAGACCTCAA-CTCGAG-TTGAGGTCTGATCTTTACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTAAAGATCAGACCTCAACTCGAGTTGAGGTCTGATCTTTACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTAAAGATCAGACCTCAACTCGAGTTGAGGTCTGATCTTTACTGC-3'

Other clones with same target sequence:

  1. TRCN0000035348