Construct: shRNA TRCN0000289414

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024045.1-960s21c1
DNA Barcode:
CGACATGTTGTGCTTGATGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DDX50 (79009)
Gene Description:
DExD-box helicase 50
Transcript:
RefSeq NM_024045.1 (NON-CURRENT)
Match location:
Position 960 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000289414)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACATGTTGTGCTTGATGAA
Hairpin Sequence:
5'-CCGG-CGACATGTTGTGCTTGATGAA-CTCGAG-TTCATCAAGCACAACATGTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACATGTTGTGCTTGATGAACTCGAGTTCATCAAGCACAACATGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACATGTTGTGCTTGATGAACTCGAGTTCATCAAGCACAACATGTCG-3'

Other clones with same target sequence:

  1. TRCN0000050992