Construct: shRNA TRCN0000289474

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012229.3-1762s21c1
DNA Barcode:
CGGTCAATTAGTGAGATTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NT5C2 (22978)
Gene Description:
5'-nucleotidase, cytosolic II
Transcript:
RefSeq NM_012229.3 (NON-CURRENT)
Match location:
Position 1762 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000289474)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTCAATTAGTGAGATTAAA
Hairpin Sequence:
5'-CCGG-CGGTCAATTAGTGAGATTAAA-CTCGAG-TTTAATCTCACTAATTGACCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTCAATTAGTGAGATTAAACTCGAGTTTAATCTCACTAATTGACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTCAATTAGTGAGATTAAACTCGAGTTTAATCTCACTAATTGACCG-3'

Other clones with same target sequence:

  1. TRCN0000050950