Construct: shRNA TRCN0000289584

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022121.4-1414s21c1
DNA Barcode:
GCTTTCCTTTAAGTGTGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PERP (64065)
Gene Description:
p53 apoptosis effector related to PMP22
Transcript:
RefSeq NM_022121.4 (NON-CURRENT)
Match location:
Position 1414 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000289584)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTCCTTTAAGTGTGAAAT
Hairpin Sequence:
5'-CCGG-GCTTTCCTTTAAGTGTGAAAT-CTCGAG-ATTTCACACTTAAAGGAAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTCCTTTAAGTGTGAAATCTCGAGATTTCACACTTAAAGGAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTCCTTTAAGTGTGAAATCTCGAGATTTCACACTTAAAGGAAAGC-3'

Other clones with same target sequence:

  1. TRCN0000123324