Construct: shRNA TRCN0000289591

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002907.3-2292s21c1
DNA Barcode:
GCACATGCTATTACTATGCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RECQL (5965)
Gene Description:
RecQ like helicase
Transcript:
RefSeq NM_002907.3 (NON-CURRENT)
Match location:
Position 2292 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000289591)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACATGCTATTACTATGCAA
Hairpin Sequence:
5'-CCGG-GCACATGCTATTACTATGCAA-CTCGAG-TTGCATAGTAATAGCATGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACATGCTATTACTATGCAACTCGAGTTGCATAGTAATAGCATGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACATGCTATTACTATGCAACTCGAGTTGCATAGTAATAGCATGTGC-3'

Other clones with same target sequence:

  1. TRCN0000052001