Construct: shRNA TRCN0000289773

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002184.2-2726s21c1
DNA Barcode:
CCAGTCCAGATATTTCACATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IL6ST (3572)
Gene Description:
interleukin 6 signal transducer
Transcript:
RefSeq NM_002184.2 (NON-CURRENT)
Match location:
Position 2726 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000289773)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTCCAGATATTTCACATT
Hairpin Sequence:
5'-CCGG-CCAGTCCAGATATTTCACATT-CTCGAG-AATGTGAAATATCTGGACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTCCAGATATTTCACATTCTCGAGAATGTGAAATATCTGGACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTCCAGATATTTCACATTCTCGAGAATGTGAAATATCTGGACTGG-3'

Other clones with same target sequence:

  1. TRCN0000058284