Construct: shRNA TRCN0000289820

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031279.3-1513s21c1
DNA Barcode:
CGAAAGTGTGACCTCTGAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ETNPPL (64850)
Gene Description:
ethanolamine-phosphate phospho-lyase
Transcript:
RefSeq NM_031279.3 (NON-CURRENT)
Match location:
Position 1513 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000289820)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAAAGTGTGACCTCTGAGAA
Hairpin Sequence:
5'-CCGG-CGAAAGTGTGACCTCTGAGAA-CTCGAG-TTCTCAGAGGTCACACTTTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAAAGTGTGACCTCTGAGAACTCGAGTTCTCAGAGGTCACACTTTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAAAGTGTGACCTCTGAGAACTCGAGTTCTCAGAGGTCACACTTTCG-3'

Other clones with same target sequence:

  1. TRCN0000034746