Construct: shRNA TRCN0000289872

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005017.2-672s21c1
DNA Barcode:
GCCCATGATGATATTCCTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PCYT1A (5130)
Gene Description:
phosphate cytidylyltransferase 1, choline, alpha
Transcript:
RefSeq NM_005017.2 (NON-CURRENT)
Match location:
Position 672 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000289872)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCATGATGATATTCCTTAT
Hairpin Sequence:
5'-CCGG-GCCCATGATGATATTCCTTAT-CTCGAG-ATAAGGAATATCATCATGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCATGATGATATTCCTTATCTCGAGATAAGGAATATCATCATGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCATGATGATATTCCTTATCTCGAGATAAGGAATATCATCATGGGC-3'

Other clones with same target sequence:

  1. TRCN0000035725