Construct: shRNA TRCN0000289930

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025208.4-1015s21c1
DNA Barcode:
GCAGAATTTGATACAGTGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDGFD (80310)
Gene Description:
platelet derived growth factor D
Transcript:
RefSeq NM_025208.4 (NON-CURRENT)
Match location:
Position 1015 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000289930)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAATTTGATACAGTGGAA
Hairpin Sequence:
5'-CCGG-GCAGAATTTGATACAGTGGAA-CTCGAG-TTCCACTGTATCAAATTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAATTTGATACAGTGGAACTCGAGTTCCACTGTATCAAATTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAATTTGATACAGTGGAACTCGAGTTCCACTGTATCAAATTCTGC-3'

Other clones with same target sequence:

  1. TRCN0000058710