Construct: shRNA TRCN0000290011

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006379.2-2525s21c1
DNA Barcode:
GCCAAGATCAACTTCAAAGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SEMA3C (10512)
Gene Description:
semaphorin 3C
Transcript:
RefSeq NM_006379.2 (NON-CURRENT)
Match location:
Position 2525 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000290011)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAGATCAACTTCAAAGTT
Hairpin Sequence:
5'-CCGG-GCCAAGATCAACTTCAAAGTT-CTCGAG-AACTTTGAAGTTGATCTTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAGATCAACTTCAAAGTTCTCGAGAACTTTGAAGTTGATCTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAGATCAACTTCAAAGTTCTCGAGAACTTTGAAGTTGATCTTGGC-3'

Other clones with same target sequence:

  1. TRCN0000058130