Construct: shRNA TRCN0000290038

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145691.3-742s21c1
DNA Barcode:
GCATCTTACAACACATGGGCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATPAF2 (91647)
Gene Description:
ATP synthase mitochondrial F1 complex assembly factor 2
Transcript:
RefSeq NM_145691.3 (NON-CURRENT)
Match location:
Position 742 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000290038)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCTTACAACACATGGGCT
Hairpin Sequence:
5'-CCGG-GCATCTTACAACACATGGGCT-CTCGAG-AGCCCATGTGTTGTAAGATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCTTACAACACATGGGCTCTCGAGAGCCCATGTGTTGTAAGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCTTACAACACATGGGCTCTCGAGAGCCCATGTGTTGTAAGATGC-3'

Other clones with same target sequence:

  1. TRCN0000045870