Construct: shRNA TRCN0000290045

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003045.4-746s21c1
DNA Barcode:
CCTACATCATCGGTACTTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC7A1 (6541)
Gene Description:
solute carrier family 7 member 1
Transcript:
RefSeq NM_003045.4 (NON-CURRENT)
Match location:
Position 746 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000290045)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTACATCATCGGTACTTCAA
Hairpin Sequence:
5'-CCGG-CCTACATCATCGGTACTTCAA-CTCGAG-TTGAAGTACCGATGATGTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTACATCATCGGTACTTCAACTCGAGTTGAAGTACCGATGATGTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTACATCATCGGTACTTCAACTCGAGTTGAAGTACCGATGATGTAGG-3'

Other clones with same target sequence:

  1. TRCN0000042965